Rabbit IgG (H+L chain) (Fab Fragment) Goat Polyclonal Antibody

CAT#: R1390HRP

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for immunoblotting (western or dot blot), ELISA, immonoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremly low background levels, absence of F(c) mediated binding, lot-to-lot consitency, high titer and specificy.
  ELISA: 1:82,000.
  Western blot: 1:1,000 - 1:5,000.
  Immunohistochemistry: 1:500 - 1:2,500.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.
  No reaction was observed against anti-Papain or anti-Goat IgG F(c).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative
  Label: HRP
  State: Lyophilized Fab fragments
  Label: Horseradish Peroxidase ; MW: 40kD)
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
  Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.