Rabbit IgG (H+L chain) (Fab Fragment) Goat Polyclonal Antibody

CAT#: R1390T

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, TRITC

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rhodamine and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: TRITC
  State: Lyophilized Fab fragments
  Label: Tetramethylrhodamine isothiocyanante ; Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 2.0 moles TRITC per mole of Goat IgG Fab
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography
• Conjugation: TRITC
• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid.
  For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.