Goat IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody

CAT#: R1392AP

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
  ELISA: 1:10,000 - 1:40,000.
  Western Blot: 1:500 - 1:5,000.
  Immunohistochemistry: 1:200 - 1:2,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Goat IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Goat IgG and Goat Serum.
  No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
• Formulation: 0.01M Tris Chloride, 0.25M Sodium Chloride; pH 8.0 with 15 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.05% (w/v) sodium azide as preservative.
  Label: AP
  State: Lyophilized F(ab')2 fragments.
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: AP
• Storage: Store the antibody as an undiluted liquid at 4°C before opening.
  DO NOT FREEZE!
  Freezing alkaline phosphatase conjugates will result
  in a substantial loss of enzymatic activity.
  Dilute only prior to immediate use.