Guinea pig IgG (H+L chain), F(ab)2 Fragment Goat Polyclonal Antibody

CAT#: R1393T

Guinea pig IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, TRITC

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and Flow Cytometry (1/500-1/2,500) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Guinea Pig IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum.
  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, and 0.01% (w/v) Sodium Azide
  Label: TRITC
  State: Lyophilized F(ab')2 fragments
  Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 1.2 moles TRITC per mole of Goat IgG F(ab')2
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: TRITC
• Storage: Store vial at 2-8°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for four weeks at 2-8°C as an undiluted liquid.
  For extended storage mix product with 50% glycerol and then aliquot contents and freeze
  at -20° C or below.
  Avoid repeated frezing and thawing.
  Dilute only prior to immediate use.