Hamster IgG (H+L chain, F(ab)2 Fragment) Rabbit Polyclonal Antibody

CAT#: R1394AP

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
  ELISA: 1:3,000 - 1:12,000.
  Western Blot: 1:500 - 1:2,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Golden Syrian Hamster IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Alkaline Phosphatase, anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum.
  No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). Limited reactivity will occur against Armenian Hamster IgG.
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) sodium azide as preservative.
  Label: AP
  State: Lyophilized F(ab')2 fragments.
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: 0.6 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: AP
• Storage: Store the antibody as an undiluted liquid at 4°C before opening.
  DO NOT FREEZE!
  Freezing alkaline phosphatase conjugates will result in a substantial
  loss of enzymatic activity.
  Dilute only prior to immediate use.