Hamster IgG (H+L chain, F(ab)2 Fragment) Rabbit Polyclonal Antibody

CAT#: R1394HRP

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (western or dot blot), ELISA, Immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  ELISA: 1:4,000 - 1:16,000.
  Western Blot: 1:500 - 1:2,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Golden Syrian Hamster IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum.
  No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). Limited reactivity will occur against Armenian Hamster IgG.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.
  Label: HRP
  State: Lyophilized F(ab')2 fragments.
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: HRP
• Storage: Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
  Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 4°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.