Hamster IgG (H+L chain, F(ab)2 Fragment) Rabbit Polyclonal Antibody

CAT#: R1394P

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Recommended Dilution: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  Recommended dilutions:
  ELISA: 1:20,000 - 1:100,000
  Western Blot: 1:2,000 - 1:10,000
  Immunohistochemistry: 1:1,000 - 1:5,000
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Golden Syrian Hamster IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Golden Syrian Hamster IgG, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum.
  No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c). Limited reactivity will occur with Armenian Hamster IgG.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid (sterile filtered) F(ab')2 fragments.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Storage: Store the antibody as an undiluted liquid at 4°C for one month or (in aliquots) at -20°C for longer.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.