Human IgG (adsorbed F(ab)2 Fragment) Goat Polyclonal Antibody

CAT#: R1396AP

Human IgG (adsorbed F(ab)2 Fragment) goat polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 is suggested for this product.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Human IgG and Human Serum.
  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Mouse and Rat Serum Proteins.
• Formulation: 0.01 M Tris Chloride, 0.15M Sodium Chloride, 0.001 M Magnesium Chloride, 0.0001 M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.05% (w/v) sodium azide as preservative.
  Label: AP
  State: Liquid (sterile filtered) F(ab')2 fragments.
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: AP
• Storage: Store the antibody as an undiluted liquid at 4°C.
  DO NOT FREEZE!
  Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  Dilute only prior to immediate use.