Human IgG (adsorbed F(ab)2 Fragment) Goat Polyclonal Antibody

CAT#: R1396F

Human IgG (adsorbed F(ab)2 Fragment) goat polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody basedfluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, hightiter and specificity.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by Immuno-electrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgG and Human Serum.
  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Mouse, Rabbit, Rat and Sheep Serum Protein
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer, IgG and Protease free.
  Label: FITC
  State: Lyophilized F(ab')2 fragments.
  Label: (Fluorescein isothiocyanate, (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: FITC
• Storage: Store vial at 4°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 4°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.