Human IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

CAT#: R1397B

Human IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Biotin

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin (cat. no. RA021HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
  A working dilution of 1/12,000 to 1/50,000 of the reconstitution concentration is suggested for this product.
  Recommended Dilutions:
  ELISA: 1/20,000-1/100,000.
  Western Blot: 1/2,000-1/10,000.
  Immunohistochemistry: 1/1,000-1/5,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG F(c) fragment
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum.
  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab')2 or Bovine, Horse, Mouse and Rat Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and protease free) and 0.01% (w/v) Sodium Azide as preservative
  Label: Biotin
  State: Lyophilized F(ab')2 fragments.
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Goat IgG molecule
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.