Human IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

CAT#: R1397R

Human IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy (1/100-1/250) and Flow Cytometry or FACS analysis (1/100-1/250) as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  The maximum amount of reagent required to stain 1 x 10⁶ cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG F(c) fragment.
• Specificity: This antibody was prepared from monospecific antiserum by Immunoaffinity Chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum.
  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab')2 or Bovine, Horse, and Mouse Serum Proteins.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: PE
  State: Lyophilized F(ab')2 fragments.
  Stabilizer: 10 mg/ml BSA (IgG and protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: R-Phycoerythrin (RPE)
  Absorption emission: 488 nm / 575 nm
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 0.5 mg/ml (by UV absorption = 82.0 at 565 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: PE
• Storage:

  Store vial at 4°C prior to restoration.
  For extended storage aliquot contents and freeze at -20°C or below.
  Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.