Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

CAT#: R1398HRP

Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  Recommended dilutions:
  ELISA 1:10,000 - 1:40,000
  Western blot 1:1,000 - 1:5,000
  Immunohistochemistry 1:500 - 1:2,500
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG F(ab')2 fragment
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgG F(ab')2 and Human Serum.
  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(c) or Bovine, Horse, Mouse and Rat Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA, IgG and Protease free, and 0.01% (w/v) Gentamicin Sulfate
  Label: HRP
  State: Lyophilized F(ab')2 fragments
  Label: Horseradish Peroxidase (Molecular Weight 40,000 daltons)
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
  Do NOT add sodium azide!
  Dilute only prior to immediate use.