Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

CAT#: R1398P

Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Application(s): suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  Recommended Dilution(s):
  ELISA: 1:25,000
  Western Blot: 1:1,000 - 1:5,000
  Immunohistochemistry: 1:500 - 1:2,500
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG F(ab')2 fragment.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to Bovine, Horse, Mouse, Rabbit and Rat Serum Proteins to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(ab')2 and Human Serum.
  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(c) or Bovine, Horse, Mouse, Rabbit and Rat Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) sodium azide as preservative.
  State: Aff - Purified
  State: Liquid (sterile filtered) F(ab')2 fragments.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.