Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) Goat Polyclonal Antibody

CAT#: R1399AP

Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-Goat Serum.
  This reagent is suitable for the detection of all human immunoglobulins and chain combinations.
  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Mouse Serum Proteins.
• Formulation: 0.01M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
  Label: AP
  State: Liquid (sterile filtered) F(ab')2 fragments
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Concentration: 0.5 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography
• Conjugation: AP
• Storage: This antibody is stable at 2-8°C as an undiluted liquid.
  DO NOT FREEZE!
  Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
  Dilute only prior to immediate use.