Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) Goat Polyclonal Antibody
CAT#: R1399B
Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, Biotin
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Specifications
Product Data | |
Recommended Dilution | ELISA: 1/20,000-1/100,000 Western Blot: 1/2,000-1/10,000 Immunohistochemistry: 1/1,000-1/5,000. This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin (Cat.-No RA021HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/50,000 of the reconstitution concentration is suggested for this product. |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Human IgG, IgA and IgM whole molecules |
Specificity | Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum. No reaction was observed against Mouse Serum proteins. This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations. |
Formulation | 0.02M Potassium Phosphate, 0.15 MSodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Biotin State: Lyophilized F(ab')2 fragments Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Goat IgG molecule |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Prepared from polyspecific antiserum by Immunoaffinity Chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |
Conjugation | Biotin |
Storage | Store vial at 2-8°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at RT. This product is stable for one month at 2-8°C as an undiluted liquid. Avoid repeated freezing and thawing. Dilute only prior to immediate use. |
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