Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) Goat Polyclonal Antibody

CAT#: R1399F

Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG, IgA, and IgM whole molecules
• Specificity: This product was prepared from polyspecific antiserum by Immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum.
  No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Mouse Serum Proteins.
  This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations.
• Formulation: 0.01M Sodium Phosphate, 0.25M Sodium Chloride, pH 7.6 with 10 mg/ml BSA (IgG and Protease free as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: FITC
  State: Lyophilized F(ab')2 fragments
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 6.0 moles FITC per mole of Goat IgG F(ab')2.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.