Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) Goat Polyclonal Antibody

CAT#: R1399HRP

Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting (Western: 1/1,000-1/10,000 or dot blot), ELISA (1/10,000-1/50,000), immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/8,000 of the reconstitution concentration is suggested for this product.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG, IgA, and IgM whole molecules
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.
  No reaction was observed against anti-Goat IgG F(c) or Mouse Serum Proteins.
  This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized F(ab')2 fragments
  Stabilizer: 10 mg/ml BSA (Ig and Protease free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: 0.5 mg/ml (by UV absorbance at 280 nm)
• Purification: Prepared from polyspecific antiserum by Immunoaffinity Chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.