Human IgM (Fc5 mu specific), F(ab)2 Fragment Goat Polyclonal Antibody

CAT#: R1400B

Human IgM (Fc5 mu specific), F(ab)2 Fragment goat polyclonal antibody, Biotin

Specifications

Product Data

• Recommended Dilution: Suitable for use in Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  ELISA: 1:10,000 - 1:50,000.
  Western Blot: 1:1,000 - 1:5,000.
  Immunohistochemistry: 1:500 - 1:3,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgM Fc5µ fragment
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgM Fc5µ and Human Serum.
  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or other human heavy or light chain proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.02% (w/v) Sodium Azide as preservative.
  Label: Biotin
  State: Lyophilized F(ab')2 fragments.
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: Biotin
• Storage: Store vial at 4°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 4°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.