Human IgM (Fc5 mu specific), F(ab)2 Fragment Goat Polyclonal Antibody

CAT#: R1400HRP

Human IgM (Fc5 mu specific), F(ab)2 Fragment goat polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/100,000.
  Western Blot: 1/2,000-1/10,000.
  Immunhistochemistry: 1/1,000-1/5,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgM Fc5µ fragment
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG or Human IgA.
  Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
• Formulation: 0.01M Sodium Phosphate, 0.25M Sodium Chloride, pH 7.6, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Thimerosal as preservative.
  Label: HRP
  State: Lyophilized F(ab)'2 fragments.
  Label: Horseradish Peroxidase (Molecular Weight 40,000 daltons)
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. Restore with deionized water or equivalent.
  Centrifuge product if not completely clear after standing at room temperature.
  This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
  Avoid cycles of freezing and thawing.