Human IgM (Fc5 mu specific), F(ab)2 Fragment Goat Polyclonal Antibody

CAT#: R1400P

Human IgM (Fc5 mu specific), F(ab)2 Fragment goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/20000-1/100000.
  Western Blot: 1/2000-1/10000.
  Immunohistochemistry: 1/1000-1/5000.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgM Fc5µ fragment
• Specificity: F(ab')2 Human IgM Fc5µ Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
  Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  State: Aff - Purified
  State: Liquid (sterile filtered) F(ab')2 fragment
  Stabilizer: None
  Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.