Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed Rabbit Polyclonal Antibody

CAT#: R1403HRP

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule
• Specificity: This antibody detects Mouse IgG (H&L) F(ab')2 Fragment (ads. to Hu serum proteins).
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free; 0.01% (w/v) Gentamicin Sulfate
  Label: HRP
  State: Lyophilized Ig fraction
  Label: Horseradish Peroxidase (Molecular Weight 40,000 daltons)
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 0.5 mg/ml (by UV absorbance at 280 nm)
• Purification: Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
• Conjugation: HRP
• Storage: Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.