Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed Rabbit Polyclonal Antibody

CAT#: R1403R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, PE

Specifications

Product Data

• Applications: FC
• Recommended Dilution: Immunofluorescence: 1/100 - 1/250
  Flow Cytometry: 1/100 - 1/250; The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
  
  Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
  Label: PE
  State: Lyophilized purified F(ab')2 Fragment
  Label: R-Phycoerythrin ( (Molecular Weight 240,000 daltons) from seaweed
  Absorption emission: 488 nm / 575 nm
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent)
• Concentration: 0.5 mg/ml (determined by absorbance = 8.2 at 565 nm for a 0.1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound)
• Purification: Immunoaffinity Chromatography.
• Conjugation: PE
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  This product is photosensitive and should be protected from light.
• Note: OD566nm/OD280nm: 2.8