Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed Rabbit Polyclonal Antibody

CAT#: R1403T

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, TRITC

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule
• Specificity: This antibody detects Mouse IgG (H&L) F(ab')2 Fragment (ads. to Hu serum proteins).
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free; 0.01% (w/v) Sodium Azide
  Label: TRITC
  State: Lyophilized Ig fraction
  Label: Tetramethylrhodamine isothiocyanate (Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 2.0 moles TRITC per mole of Rabbit IgG F(ab')2
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 0.5 mg/ml (by UV absorbance at 280 nm)
• Purification: Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
• Conjugation: TRITC
• Storage: Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below. For extended storage add glycerol to 50%.
  Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.