Mouse IgG (H+L chain), F(ab)2 Fragment Goat Polyclonal Antibody

CAT#: R1404AP

Mouse IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, AP


USD 250.00

3 Days*

Size
    • 500 ug

Product Images

Specifications

Product Data
Recommended Dilution Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilution(s): This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/600 to 1/2,750 of the reconstitution concentration is suggested for this product.
Host Goat
Clonality Polyclonal
Immunogen Mouse IgG whole molecule.
Specificity This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat
Serum, Mouse IgG and Mouse Serum.
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c).
Formulation 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0 containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
Label: AP
State: Liquid (sterile filtered) F(ab')2 fragments.
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Concentration 0.6 mg/ml (by UV absorbance at 280 nm).
Purification Immunoaffinity Chromatography.
Conjugation AP
Storage Store the antibody undiluted at 2-8°C
DO NOT FREEZE.
Dilute only prior to immediate use.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.