Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

CAT#: R1406F

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, FITC

Specifications

Product Data

• Applications: ELISA, IF
• Recommended Dilution:
  This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
  Recommended Dilutions:
  

  FLISA: 1/10,000-1/50,000.
  IF Microscopy: 1/1,000-1/5,000.
  Flow Cytometry: 1/500-1/2,500.

  
  
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG F(c) fragment.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.
  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab')2 or Bovine, Horse and Human Serum Proteins.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: FITC
  State: Lyophilized F(ab')2 fragments
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 4.1 moles FITC per mole of Goat IgG F(ab')2.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
• Conjugation: FITC
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.