Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

CAT#: R1406HRP

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/20000.
  Western Blot: 1/500-1/2000.
  Immunohistochemistry: 1/250-1/1000.
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG F(c) fragment.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.
  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab’)2 or Bovine, Horse, and Human Serum Proteins.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized F(ab')2 fragments
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Horseradish Peroxidase (Molecular Weight 40,000 daltons)
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
• Conjugation: HRP
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Note: Do Not add Sodium Azide!