Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

CAT#: R1406P

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/10,000.
  Western Blot : 1/2,000-1/10,000
  Immunohistochemistry: 1/1,000-1/5,000.
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG F(c) fragment.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.
  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab’)2 or Bovine, Horse and Human Serum Proteins.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  State: Aff - Purified
  State: Liquid (sterile filtered) purified F(ab')2 fragments
  Stabilizer: None
  Preservative: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.