Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

CAT#: R1406T

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, TRITC

Specifications

Product Data

• Applications: ELISA, IF
• Recommended Dilution:
  This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000.
  IF Microscopy: 1/1,000-1/5,000.
  Flow Cytometry: 1/500-1/2,500.
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG F(ab')2 fragment.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.
  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab')2 or Bovine, Horse and Human Serum Proteins.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: TRITC
  State: Lyophilized F(ab')2 fragments
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 4.0 moles TRITC per mole of Goat IgG F(ab')2).
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
• Conjugation: TRITC
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.