Mouse IgM (Mu chain specific), F(ab)2 Fragment Goat Polyclonal Antibody

CAT#: R1408P

Mouse IgM (Mu chain specific), F(ab)2 Fragment goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Recommended Dilution: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions:
  ELISA: 1:2,000-1:10,000.
  Western Blot: 1:200-1:2,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgM mu heavy chain.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum.
  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
  Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid (sterile filtered) F(ab')2 fragments.
• Concentration: 1.15 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.