Mouse IgM (Mu chain specific), F(ab)2 Fragment Goat Polyclonal Antibody

CAT#: R1408T

Mouse IgM (Mu chain specific), F(ab)2 Fragment goat polyclonal antibody, TRITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy (1:50-1:200) and Flow Cytometry (1:50-1:200) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgM mu chain.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum.
  No reaction was observed against anti-Pepsin or anti- Goat IgG F(c). Specificty was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.05% (w/v) Sodium Azide as preservative.
  Label: TRITC
  State: Lyophilized F(ab')2 fragments.
  Label: Tetramethylrhodamine isothiocyanante ; Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 2.1 moles TRITC per mole of Goat IgG F(ab')2).
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: TRITC
• Storage: Store vial at 2-8°C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.