Mouse IgA (H+L chain), F(ab)2 Fragment Goat Polyclonal Antibody

CAT#: R1409HRP

Mouse IgA (H+L chain), F(ab)2 Fragment goat polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000-1/5,000 of the reconstitution concentration is suggested for this product.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG, IgA and IgM whole molecules.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.
  No reaction was observed against anti-Goat IgG F(c). This reagent is suitable for the detection of all mouse subclasses, isotypes and chain combinations.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA(IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.
  Label: HRP
  State: Lyophilized F(ab')2 fragments.
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. Restore with 0.5 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.