Rabbit IgG (H+L chain) F(ab)2 Fragment Goat Polyclonal Antibody

CAT#: R1410B

Rabbit IgG (H+L chain) F(ab)2 Fragment goat polyclonal antibody, Biotin


USD 255.00

3 Days*

Size
    • 500 ug

Product Images

Specifications

Product Data
Recommended Dilution Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody
based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:9,000 to 1:36,000 is suggested for this product.
Host Goat
Clonality Polyclonal
Immunogen Rabbit IgG whole molecule
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA, IgG and Protease free, and 0.01% (w/v) Sodium Azide
Label: Biotin
State: Lyophilized F(ab')2 fragments
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Molar radio: 10-20 BAC molecules per Goat IgG molecule
Reconstitution Method Restore with 0.5 ml of deionized water or equivalent.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
Conjugation Biotin
Storage Store vial at 2-8°C prior to restoration.
Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
Dilute only prior to immediate use.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.