Rabbit IgG (F(ab)2 specific adsorbed F(ab)2 Fragment) Goat Polyclonal Antibody

CAT#: R1412P

Rabbit IgG (F(ab)2 specific adsorbed F(ab)2 Fragment) goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Recommended Dilution: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  Recommended dilutions:
  ELISA 1:10,000-1:50,000
  Western blot 1:1,000-1:5,000
  Immunohistochemistry 1:500-1:3,000
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG F(ab')2 fragment.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab')2 and Rabbit Serum.
  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
• Formulation: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.4, containing 0.01% (w/v) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid (sterile filtered) F(ab')2 fragments.
• Concentration: 0.5 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Storage: This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage aliquot contents and freeze at -20°C or below.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.