Rat IgG (H+L chain), F(ab)2 Fragment Rabbit Polyclonal Antibody

CAT#: R1413P

Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Recommended Dilution: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  Recommende Dilutions:
  ELISA: 1/20,000-1/100,000
  Western Blot: 1/2,000-1/10,000
  Immunohistochemistry: 1/1,000-1/5,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Rat IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgG and Rat Serum.
  No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit & Sheep Serum Protein
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid (sterile filtered) F(ab')2 fragments.
• Concentration: 2.72 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.