Rat IgG (H+L chain), F(ab)2 Fragment Rabbit Polyclonal Antibody

CAT#: R1413TR

Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Texas Red

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Rat IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgG and Rat Serum.
  No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
• Formulation: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: Texas Red
  State: Lyophilized F(ab')2 fragments.
  Label: -- Sulfonyl Chloride (TR; Molecular Weight 625 daltons)
  Absorption emission: 596 nm / 620 nm
  Molar radio: 3.8 moles Texas Red TM per mole of Rabbit IgG F(ab')2.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: Texas Red
• Storage: Store vial at 2-8°C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.