Rat IgG (F(ab)2 Fragment) Fc specific Goat Polyclonal Antibody

CAT#: R1414P

Rat IgG (F(ab)2 Fragment) Fc specific goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Recommended Dilution: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  Recommended Dilution(s):
  ELISA: 1/10,000 - 1/50,000.
  Western Blot: 1/2,000 - 1/5,000.
  Immunohistochemistry: 1/1,000 - 1/3,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rat IgG F(c) fragment
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum.
  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab')2 or Bovine, Horse and Human Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid (sterile filtered) F(ab')2 fragments.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.