Mouse IgM (Mu chain specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

CAT#: R1426R

Mouse IgM (Mu chain specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.
  Recommended dilutions:
  Flow cytometry 1:100 - 1:250
  IF microscopy 1:100 - 1:250
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgM mu heavy chain
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgM, and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or human, bovine or horse serum proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride; pH 7.2, with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.02% (w/v) Sodium Azide as preservative
  Label: PE
  State: Lyophilized
  Label: R-Phycoerythrin ( (Molecular Weight 240,000 daltons) from seaweed
  Absorption emission: 490 nm, 545 nm and 565 nm / 580 nm
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent)
• Concentration: 0.5 mg/ml
• Purification: F(ab')2 fragment of affinity purified antibody
• Conjugation: PE
• Storage: Store vial at 2-8°C prior to opening. DO NOT FREEZE. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. After restoration, this product is stable at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date for properly stored material is six (6) months from date of opening.