Rabbit IgG F(ab)2 Fragment (H+L chain) Donkey Polyclonal Antibody

CAT#: R1427R

Rabbit IgG F(ab)2 Fragment (H+L chain) donkey polyclonal antibody, PE

Specifications

Product Data

• Applications: FC
• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions:
  Immunofluorescence: 1/100-1/200.
  Flow Cytometry: 1/100-1/20. The maximum amount of reagent required to stain 1 x 10e6 cells is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
  As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
• Reactivities: Rabbit
• Host: Donkey
• Clonality: Polyclonal
• Immunogen: Rabbit IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
  No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride; pH 7.2
  Label: PE
  State: Lyophilized F(ab')2 fragments.
  Stabilizer: 10 mg/ml BSA (Immunoglobulin and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: R-Phycoerythrin ( Molecular Weight 240,000 daltons)
  Absorption emission: 488 nm / 575 nm
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: PE
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  This product is photosensitive and should be protected from light.