Rabbit IgG (H+L chain, adsorbed) Goat Polyclonal Antibody

CAT#: R1454AP

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended dilutions:
  ELISA: 1:3,000 - 1:13,500.
  Western blot 1:500 - 1:2,500.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG whole molecule
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free and 0.01% (w/v) Sodium Azide
  Label: AP
  State: Liquid (sterile filtered)
  Label: Alkaline Phosphatase (Calf Intestine, Molecular Weight 140,000 daltons))
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: AP
• Storage: Store vial at 4°C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.