Rabbit IgG (H+L chain, adsorbed) Goat Polyclonal Antibody

CAT#: R1454P

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
  ELISA: 1/15,000-1/30,000
  Western blot: 1/500-1/2,000
  Immunohistochemistry: 1/500-1/2,000.
• Reactivities: Rabbit
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG whole molecule in goat.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. Specificity was confirmed by ELISA at less than 0.1 % of target signal.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid (sterile filtered) purified Ig fraction.
  Stabilizer: None
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.