Sheep IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1456AP

Sheep IgG (H+L chain) rabbit polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/25,000.
  Western Blot: 1/500-1/2,000.
  Immunohistochemistry: 1/200-1/1,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Sheep IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Sheep IgG and Sheep Serum.
• Formulation: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001 M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0 containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
  Label: AP
  State: Liquid (sterile filtered) purified Ig fraction.
  Label: Alkaline Phosphatase (Calf Intestine) (MW. 140,000 daltons)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: AP
• Storage: Store the antibody undiluted at 2-8°C before opening.
  DO NOT FREEZE!
  Dilute only prior to immediate use.
  Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  Avoid repeated freezing and thawing.