Sheep IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1456B

Sheep IgG (H+L chain) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Recommended Dilution: Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using peroxidase conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature.
  A working dilution of 1:20,000 to 1:80,000 is suggested for this product.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Sheep IgG whole molecule
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Sheep IgG, and Sheep Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% Sodium Azide as preservative
  Label: Biotin
  State: Lyophilized purified IgG fraction
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Rabbit IgG molecule
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml ( by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration. Restore with deionized water. Centrifuge product if not completely clear after standing at room temperature.
  This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
  Avoid cycles of freezing and thawing.