Sheep IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1456F

Sheep IgG (H+L chain) rabbit polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000.
  IF Microscopy: 1/1,000-1/5,000.
  Flow Cytometry: 1/500-1/2,500.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Sheep IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG and Sheep Serum.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: FITC
  State: Lyophilized purified IgG fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 3.3 moles FITC per mole of Rabbit IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.2 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20°C or below.
  Centrifuge product if not completely clear after standing at room temperature.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.