Sheep IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1456T

Sheep IgG (H+L chain) rabbit polyclonal antibody, TRITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Sheep IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG and Sheep Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: TRITC
  State: Lyophilized purified Ig fraction.
  Label: Tetramethylrhodamine isothiocyanante ; Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 3.2 moles TRITC per mole of Rabbit IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: TRITC
• Storage: Store vial at 2-8°C prior to restoration.
  For extended storage mix with Glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.