Sheep IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1456TR

Sheep IgG (H+L chain) rabbit polyclonal antibody, Texas Red

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Sheep IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG and Sheep Serum.
  No reaction was observed against human serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide
  Label: Texas Red
  State: Lyophilized IgG fraction
  Label: (TM) Sulfonyl Chloride (Molecular Weight 625 daltons)
  Absorption emission: 596 nm / 620 nm
  Molar radio: 2.3 moles Texas Red (TM) per mole of Rabbit IgG
• Reconstitution Method: Restore with 2.0 ml of deionized water or equivalent.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography
• Conjugation: Texas Red
• Storage: Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for four weeks at 4°C as an undiluted liquid.
  For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
  Dilute only prior to immediate use.