Mouse IgG + IgM (H+L chain), adsorbed Goat Polyclonal Antibody

CAT#: R1469AP

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or dot blot, 1/500-1/2,500), ELISA (1/3,000-1/12,000) and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG and IgM whole molecules
• Specificity: This product was prepared from polyspecific antiserum by Immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, Mouse IgG and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins.
• Formulation: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001 M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: AP
  State: Liquid (sterile filtered) purified IgG fraction.
  Label: Alkaline Phosphatase (Calf Intestine) (MW 140,000 daltons)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: AP
• Storage: Store vial at 2-8°C before opening.
  DO NOT FREEZE!.
  This product is stable at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
  Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  Avoid repeated freezing and thawing.