Mouse IgG + IgM (H+L chain), adsorbed Goat Polyclonal Antibody

CAT#: R1469B

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, Biotin


USD 255.00

3 Days*

Size
    • 1 mg

Product Images

Specifications

Product Data
Recommended Dilution Suitable for Immunoblotting (Western Blot, 1:2,000-1:10,000), ELISA (1:20,000-1:100,000), Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Host Goat
Clonality Polyclonal
Immunogen Mouse IgG and IgM whole molecules
Specificity This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Biotin, Mouse IgG and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Label: Biotin
State: Lyophilized purified IgG fraction.
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Molar radio: 10-20 BAC molecules per bbb IgG molecule.
Reconstitution Method Restore with 1.0 ml of deionized water (or equivalent).
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity chromatography.
Conjugation Biotin
Storage Store the antibody at 2-8°C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20° C or below. Centrifuge product if not completely clear after standing at room
temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
Avoid repeated freezing and thawing.
Dilute only prior to immediate use.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.