Mouse IgG (Fc specific) Goat Polyclonal Antibody

CAT#: R1612AP

Mouse IgG (Fc specific) goat polyclonal antibody, AP

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/2,000-1/100,000
  Western blot: 1/500-1/2,500
  Immunohistochemistry: 1/200-1/1,000.
  Note: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code #NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/5,000 of the reconstitution concentration is suggested for this product.
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG F(c) fragment
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.
  No reaction was observed against Mouse IgG F(ab')2.
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0 with 10 mg/ml BSAm (Immunoglobulin and Protease free) and 0.01% Sodium Azide.
  Label: AP
  State: Liquid (sterile filtered) purified IgG fraction.
  Label: Alkaline Phosphatase (Calf Intestine)
• Concentration: 0.81 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities.
• Conjugation: AP
• Storage: Store vial at 4°C prior to restoration.
  DO NOT FREEZE!
  This product is stable for one month at 2-8°C as an undiluted liquid.
  Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.