Mouse IgG (Fc specific) Goat Polyclonal Antibody

CAT#: R1612B

Mouse IgG (Fc specific) goat polyclonal antibody, Biotin


USD 265.00

3 Days*

Size
    • 2 mg

Product Images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommended Dilution ELISA: 1/20,000-1/100,000
Western blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:130,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Host Goat
Clonality Polyclonal
Immunogen Anti-mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in goat.
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.
No reaction was observed against Mouse IgG F(ab’)2.
Formulation 0.01M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (Immunoglobulin and Protease free) and 0.01% Thimerisol.
Label: Biotin
State: Lyophilized purified IgG fraction.
Reconstitution Method Restore with 1.0 ml of deionized water (or equivalent).
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities.
Conjugation Biotin
Storage Store vial at 4°C prior to restoration.
For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.