Mouse IgG (Fc specific) Goat Polyclonal Antibody

CAT#: R1612HRP

Mouse IgG (Fc specific) goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000
  Western blot: 1/1,000-1/5,000
  Flow Cytometry: 1/500-1/2,500
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG F(c) fragment
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab')2 fragment.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified Ig fraction.
  Stabilizer: 10 mg/ml BSA (Immunoglobulin and Protease free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 mL of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: HRP
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C or below for longer.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.